Spec-Chem Industry Inc. Efficacy Test Center recently applied HPLC method to evaluate function of SpecKare EyeS100 as ACE-Inhibitors.
The test proved that SpecKare EyeS100 works effectively as an ACE inhibitor.
Angiotensin converting enzyme (ACE) plays an important role in regulating blood pressure in body. Hippuric acid produced by the incubation system of N-Hippuryl-His-Leu hydrate and ACE is used to evaluate inhibitory activity towards ACE from different samples.
Hippuric acid has a maximum absorption at 228 nm. Therefore, the inhibitory activity on ACE could be evaluated by measuring the amount of hippuric acid produced by high performance liquid chromatography (HPLC).
|Sample||Concentration (%)||ACE inhibitory activity (%)|
|SpecKare EyeS100||0.5% wt.||73.2%|
|SpecKare EyeS100||1% wt.||78.0%|
|SpecKare EyeS100||3% wt.||81.4%|
|SpecKare EyeS100||5% wt.||85.5%|
|SpecKare EyeS100||10% wt.||87.3%|